Date Published:
2006 Dec 1
Publication Type:
Journal Article
Authors:
Secondary:
Drug Alcohol Depend
Volume:
85
Pagination:
236-43
Issue:
3
PMID:
16766138
URL:
https://pubmed.ncbi.nlm.nih.gov/16766138
DOI:
10.1016/j.drugalcdep.2006.05.013
Keywords:
Adolescent;Adult;Cell Proliferation;Cocaine-Related Disorders;Cytokines;Female;Hepatitis B;Hepatitis C;Heroin Dependence;HIV Infections;Humans;Leukocytes, Mononuclear;Male;Th1 Cells;Th2 Cells
Abstract:
<p>HCV-infected "speedball" users (n = 30) were selected from an original cohort of 400 intravenous drug users for cytokine analysis. Cytokine concentrations (TNF-alpha, IL-1beta, IL-6, IFN-gamma, IL-2, IL-4, IL-10 and IL-12) were determined in plasma and peripheral blood mononuclear cells (PBMC) cultures derived ex vivo from these patients. In addition, lymphocyte proliferation was measured in 49 HCV-positive "speedball" users. TNF-alpha, IL-6, IFN-gamma, IL-2, IL-4, IL-10, IL-12 cytokines and not IL-1beta were significantly increased in plasma from HCV-positive "speedball" users compared with healthy controls. Except for IL-10, all other cytokines measured were augmented in phytohemagglutinin-stimulated PBMC cultures from HCV-positive "speedball" users. Likewise, overproduction of cytokines TNF-alpha, IL-1beta, IL-6 and IFN-gamma, was consistently detected when PBMC cultures from HCV-positive "speedball" users were stimulated with a biological response modifier. However, HCV-infected "speedball" users showed significant reduction in lymphoproliferative activity. Compared with healthy subjects, there was a consistent overproduction of both TH1 and TH2 type cytokines in the plasma and PBMC's of HCV-infected "speedball" users. Furthermore, there was a persistent reduction of lymphoproliferative activity in this group. These immunologic abnormalities, coupled with the range of response between the two TH-types in HCV-infected "speedball" users, suggest impairment in the regulatory mechanism of the TH1-TH2 system.</p>