Date Published:
2017 11

Publication Type:
Journal Article

Authors:

M. Veenstra
D.W. Williams
T.M. Calderon
K. Anastos
S. Morgello
J.W. Berman

Secondary:
J Leukoc Biol

Volume:
102

Pagination:
1173-1185

Issue:
5

PMID:
28754798

URL:
https://pubmed.ncbi.nlm.nih.gov/28754798

DOI:
10.1189/jlb.3HI0517-167R

Keywords:
Adult;Antiretroviral Therapy, Highly Active;Astrocytes;Blood-Brain Barrier;CD4 Lymphocyte Count;CD4-Positive T-Lymphocytes;Cognitive Dysfunction;Endothelial Cells;Female;Gene Expression;GPI-Linked Proteins;HIV Infections;HIV-1;Humans;Immunologic Factors;Lipopolysaccharide Receptors;Male;Middle Aged;Models, Biological;Monocytes;Primary Cell Culture;Receptors, CXCR;Receptors, IgG;Transendothelial and Transepithelial Migration;Viral Load

Abstract:
<p>CD14CD16 monocytes transmigrate into the CNS of HIV-positive people in response to chemokines elevated in the brains of infected individuals, including CXCL12. Entry of these cells leads to viral reservoirs, neuroinflammation, and neuronal damage. These may eventually lead to HIV-associated neurocognitive disorders. Although antiretroviral therapy (ART) has significantly improved the lives of HIV-infected people, the prevalence of cognitive deficits remains unchanged despite ART, still affecting >50% of infected individuals. There are no therapies to reduce these deficits or to prevent CNS entry of CD14CD16 monocytes. The goal of this study was to determine whether CXCR7, a receptor for CXCL12, is expressed on CD14CD16 monocytes and whether a small molecule CXCR7 antagonist (CCX771) can prevent CD14CD16 monocyte transmigration into the CNS. We showed for the first time that CXCR7 is on CD14CD16 monocytes and that it may be a therapeutic target to reduce their entry into the brain. We demonstrated that CD14CD16 monocytes and not the more abundant CD14CD16 monocytes or T cells transmigrate to low homeostatic levels of CXCL12. This may be a result of increased CXCR7 on CD14CD16 monocytes. We showed that CCX771 reduced transmigration of CD14CD16 monocytes but not of CD14CD16 monocytes from uninfected and HIV-infected individuals and that it reduced CXCL12-mediated chemotaxis of CD14CD16 monocytes. We propose that CXCR7 is a therapeutic target on CD14CD16 monocytes to limit their CNS entry, thereby reducing neuroinflammation, neuronal damage, and HIV-associated neurocognitive disorders. Our data also suggest that CCX771 may reduce CD14CD16 monocyte-mediated inflammation in other disorders.</p>