OTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis

TitleOTK18 expression in brain mononuclear phagocytes parallels the severity of HIV-1 encephalitis
Publication TypeJournal Article
Year of Publication2004
AuthorsCarlson, KA, Limoges, J, Pohlman, GD, Poluektova, LY, Langford, D, Masliah, E, Ikezu, T, Gendelman, HE
JournalJournal of Neuroimmunology
Volume150
Issue1-2
Pagination186-98
Date Published05/2004
KeywordsCells, Cultured, DNA-Binding Proteins, Encephalitis, External, Organ Specificity, Transcription Factors, Viral
Abstract

OTK18 was isolated by mRNA differential display of human monocyte-derived macrophages (MDM) infected with human immunodeficiency virus type one (HIV-1). Northern blot and real-time reverse transcription polymerase chain reaction showed low levels of OTK18 expression in human tissue, which markedly increased during advanced HIV-1 encephalitis (HIVE). Immunocytochemistry, using rabbit polyclonal antisera, showed OTK18 localized to brain mononuclear phagocytes (MP) in moderate to severe HIV-1 encephalitis. OTK18 expression was selective and not found in HIV-1-infected brain tissue with limited neuropathological abnormalities, nor in cytomegalovirus encephalitis, multiple sclerosis, Alzheimer's disease, or uninfected control brains. Thus, OTK18 expression in brain mononuclear phagocytes is a signature for advanced HIV-1 encephalitis.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/15081264