Identification, Localization, and Quantification of HIV Reservoirs Using Microscopy.

TitleIdentification, Localization, and Quantification of HIV Reservoirs Using Microscopy.
Publication TypeJournal Article
Year of Publication2019
AuthorsPrevedel, L, Ruel, N, Castellano, P, Smith, C, Malik, S, Villeux, C, Bomsel, M, Morgello, S, Eugenin, EA
JournalCurr Protoc Cell Biol
Volume82
Issue1
Paginatione64
Date Published03/2019
ISSN1934-2616
Abstract

The major barrier to eradicating human immunodeficiency virus-1 (HIV) infection is the generation and extended survival of HIV reservoirs. In order to eradicate HIV infection, it is essential to detect, quantify, and characterize circulating and tissue-associated viral reservoirs in infected individuals. Currently, PCR-based technologies and Quantitative Viral Outgrowth Assays (Q-VOA) are the gold standards to detect viral reservoirs. However, these methods are limited to detecting circulating viral reservoirs, and it has been shown that they misrepresent the size of the reservoirs, largely because they detect only one component of the HIV life cycle and are unable to detect viral reservoirs in tissues. Here, we described the use of multiple detection systems to identify integrated HIV DNA or viral mRNA and several HIV proteins in circulating and tissue reservoirs using improved staining and microscopy techniques. We believe that this imaging-based approach for detecting HIV reservoirs will lead to breakthroughs necessary to eradicate these reservoirs. © 2018 by John Wiley & Sons, Inc.

DOI10.1002/cpcb.64
Alternate JournalCurr Protoc Cell Biol
PubMed ID30265439
PubMed Central IDPMC6386609
Grant ListR01 MH096625 / MH / NIMH NIH HHS / United States
R01 NS105584 / NS / NINDS NIH HHS / United States
U24 MH100931 / MH / NIMH NIH HHS / United States