CD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis

TitleCD45 isoform expression in microglia and inflammatory cells in HIV-1 encephalitis
Publication TypeJournal Article
Year of Publication2006
AuthorsCosenza-Nashat, MA, Kim, MO, Zhao, ML, Suh, H-S, Lee, SC
JournalBrain Pathology (Zurich, Switzerland)
Volume16
Issue4
Pagination256-65
Date Published10/2006
KeywordsAIDS Dementia Complex, Antigens, Brain, CD45, External, HIV-1, Humans, Immunohistochemistry, Lymphocytes, Macrophages, Microglia, Middle Aged, Protein Isoforms
Abstract

CD45 is a membrane tyrosine phosphatase that modulates the function of the hematopoietic cells. In vitro, agonist antibodies to CD45RO or CD45RB isoforms have been shown to suppress microglial activation, but whether microglia in vivo express these isoforms in HIV encephalitis (HIVE) is unknown. Brain sections from control and HIVE were immunostained for CD45 isoforms using exon-specific antibodies (RA, RB, RC and RO). RA and RC were limited to rare lymphocytes, while RB expression was robust in microglia and inflammatory cells. RO was low in control microglia, but increased in HIVE. RO was also localized to macrophages and CD8+ T cells. Targeting CD45 in vivo with isoform-specific antibodies remains a therapeutic option for neuroinflammatory diseases.

URLhttp://www.ncbi.nlm.nih.gov/pubmed/17107594